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EXP001744

Paper

Renal-targeting peptide-microRNA nanocomplex for near IR imaging and therapy of renal ischemia/reperfusion injury (2021)

Peptide

FA-R9-FPcas-3 (FMN-17 carrier peptide)

Sequence: FA-(Arg)9-(click-linker)-Ac-DEVD-PABA-mCy (NIR dye)

RNA

miRNA mimic

All experiment fields

Experiment Id EXP001744
Paper Renal-targeting peptide-microRNA nanocomplex for near IR imaging and therapy of renal ischemia/reper
Peptide FA-R9-FPcas-3 (FMN-17 carrier peptide)
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect no
Endosomal Escape Evidence
Peptide Concentration Matched to FMN-17 conditions
Rna Concentration 100 nM miR-NC
Mixing Ratio Matched to FMN-17 (e.g., 20:1)
Formulation Format Self-assembled peptide/miRNA nanocomplex (FMN)
Formulation Components FA-R9-FPcas-3 peptide + miR-NC (self-assembled FMN-NC)
Size Nm 200.00
Zeta Mv 5.00
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells HK-2 (H/R model)
Animal Model
Administration Route
Output Type in vitro negative control
Output Value Does not reduce H/R-induced apoptosis or cleaved caspase-3 compared with FMN-17; higher fluorescence turn-on signal consistent with higher caspase-3 activity.
Output Units
Output Notes Used as control nanocomplex (FMN-NC) in apoptosis/ROS/NAG/TUNEL and imaging readouts.
Toxicity Notes
Curation Notes