FA-R9-FPcas-3 (FMN-17 carrier peptide)
Sequence: FA-(Arg)9-(click-linker)-Ac-DEVD-PABA-mCy (NIR dye)
| Experiment Id | EXP001745 |
|---|---|
| Paper | Renal-targeting peptide-microRNA nanocomplex for near IR imaging and therapy of renal ischemia/reper |
| Peptide | FA-R9-FPcas-3 (FMN-17 carrier peptide) |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | FMN-17 prepared at peptide:miRNA ratios ~20:1 (as used for characterization/assembly) |
| Formulation Format | Self-assembled peptide/miRNA nanocomplex (FMN) |
| Formulation Components | FA-R9-FPcas-3 peptide + miR-17-5p (FMN-17); Cy5-labeled miRNA for imaging variants |
| Size Nm | 200.00 |
| Zeta Mv | 5.00 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | C57BL/6 mice renal ischemia–reperfusion injury (bilateral renal pedicle clamping) |
| Administration Route | Intravenous (tail vein) injection |
| Output Type | in vivo functional delivery + imaging |
| Output Value | In C57BL/6 renal ischemia–reperfusion injury model, FMN-17 selectively accumulated in kidneys (Cy5 imaging) and alleviated renal injury (H&E/PAS/Masson), reduced cleaved caspase-3, ROS and NAG, and reduced TUNEL-positive apoptotic cells; lower in vivo NIR fluorescence signal over time vs control indicates reduced caspase-3 activation. |
| Output Units | |
| Output Notes | RIRI induced by bilateral renal pedicle clamping (45 min) followed by reperfusion; blood and kidney collected 24 h after I/R. FMN-17-Cy5 injected i.v. via tail vein for biodistribution imaging; ex vivo organ imaging showed kidney-dominant signal. |
| Toxicity Notes | Reported ALT/AST testing and H&E assessment of organs; no major toxicity signals described under study conditions. |
| Curation Notes |