Liposomes Incorporating Transferrin and Stearic Acid-modified Octa-arginine for siRNA Delivery (2017)
Stearic acid–modified octa-arginine (StA-R8)
Sequence: Stearyl-(Arg)8 ; R8 = RRRRRRRR
| Experiment Id | EXP001747 |
|---|---|
| Paper | Liposomes Incorporating Transferrin and Stearic Acid-modified Octa-arginine for siRNA Delivery |
| Peptide | Stearic acid–modified octa-arginine (StA-R8) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | StA-R8:ePC:cholesterol molar ratio 20:35:45 in ethanol; liposomes formed by ethanol injection into HEPES buffer; siRNA complexation 20 min RT. |
| Rna Concentration | Not explicitly stated for functional assay; uptake assays use FAM-siRNA (dose not specified in abstract; typical in vitro). |
| Mixing Ratio | N/P ratio tested 0.5–8; functional/uptake complexes prepared at N/P=6. |
| Formulation Format | CPP-incorporated liposomes complexed with siRNA (lipoplex via electrostatic complexation) |
| Formulation Components | SLP/siRNA: liposomes containing StA-R8 + ePC + cholesterol; siRNA complexed at N/P=6 |
| Size Nm | 120.10 |
| Zeta Mv | 7.70 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa; HepG2 |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro uptake + gene silencing (protein) |
| Output Value | Survivin protein reduced to 44.1% of untreated control (Western blot, HeLa) after SLP/siRNA treatment. |
| Output Units | |
| Output Notes | Flow cytometry (FAM-siRNA) shows much higher uptake vs naked siRNA in HeLa and HepG2. Western blot: 4 h serum-free incubation with lipoplex then 44 h in 10% FBS before harvest. |
| Toxicity Notes | MTT: SLP showed reduced cytotoxicity vs free StA-R8; cell viability >85% across tested concentrations in HeLa and HepG2. |
| Curation Notes |