Liposomes Incorporating Transferrin and Stearic Acid-modified Octa-arginine for siRNA Delivery (2017)
Stearic acid–modified octa-arginine (StA-R8)
Sequence: Stearyl-(Arg)8 ; R8 = RRRRRRRR
| Experiment Id | EXP001748 |
|---|---|
| Paper | Liposomes Incorporating Transferrin and Stearic Acid-modified Octa-arginine for siRNA Delivery |
| Peptide | Stearic acid–modified octa-arginine (StA-R8) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Tf-PEG-DSPE post-inserted onto preformed SLP/siRNA by incubation at 37°C for 30 min. |
| Rna Concentration | Not explicitly stated for functional assay; FAM-siRNA used for uptake assays. |
| Mixing Ratio | TSLP/siRNA prepared at N/P=6 (as used in gel/uptake/efficacy). |
| Formulation Format | CPP-incorporated liposomes complexed with siRNA (lipoplex via electrostatic complexation) |
| Formulation Components | TSLP/siRNA: SLP/siRNA with transferrin-PEG-DSPE post-inserted (Tf-conjugated StA-R8 liposomes); N/P=6 |
| Size Nm | 148.70 |
| Zeta Mv | 4.70 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa; HepG2 |
| Animal Model | |
| Administration Route | |
| Output Type | in vitro uptake + gene silencing (protein) |
| Output Value | Survivin protein reduced to 20.4% of untreated control (Western blot, HeLa) after TSLP/siRNA treatment; higher uptake vs SLP/siRNA in HeLa and HepG2 by flow cytometry. |
| Output Units | |
| Output Notes | Tf conjugation improved delivery efficiency vs non-conjugated SLP while maintaining low cytotoxicity. |
| Toxicity Notes | MTT: TSLP viability >85% across tested concentrations; similar to SLP (lower than free StA-R8). |
| Curation Notes |