On the Cellular Uptake and Membrane Effect of the Multifunctional Peptide, TatLK15 (2013)
Sequence: RKKRRQRRRGGGKLLKLLLKLLLKLLK-CONH2
| Experiment Id | EXP001750 |
|---|---|
| Paper | On the Cellular Uptake and Membrane Effect of the Multifunctional Peptide, TatLK15 |
| Peptide | TatLK15 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | Dose-dependent uptake/toxicity tested (subcritical vs critical dose; time-dependent up to ~3 h low toxicity below threshold). |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | Free peptide uptake/toxicity study (no RNA payload in this paper). |
| Formulation Components | TatLK15 or Tat peptide alone; fluorescent labeling used for uptake quantification (flow cytometry/confocal; high-content screening). |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Multiple cell lines including K562 leukemia and others (uptake mechanism and toxicity compared across lines). |
| Animal Model | |
| Administration Route | |
| Output Type | cellular uptake + membrane/toxicity effects (not RNA delivery) |
| Output Value | Dose- and cell line-dependent uptake; uptake influenced by membrane potential; toxicity limited below a critical dose for ~3 h but rapid at higher doses. |
| Output Units | |
| Output Notes | Study characterizes TatLK15 secondary structure (LK15 helicity retained with Gly bridge) and cellular uptake/toxicity mechanisms; RNA delivery referenced only as prior work. |
| Toxicity Notes | Assessed by LDH release, MTT, hemolysis, and time-dependent membrane-impermeant dye uptake (high-content screening). |
| Curation Notes |