Sequence: GRKKRRQRRRPPQ (with C-terminal Cys in construct: ...PPQC)
| Experiment Id | EXP001770 |
|---|---|
| Paper | Lung Delivery Studies Using siRNA Conjugated to TAT(48-60) and Penetratin Reveal Peptide Induced Red |
| Peptide | TAT(48-60) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Up to 10 µM (peptide–siRNA conjugate) |
| Rna Concentration | Up to 10 µM (siRNA duplex as conjugate) |
| Mixing Ratio | covalent conjugate (N/A) |
| Formulation Format | disulfide-linked peptide–siRNA conjugate |
| Formulation Components | CPP (TAT(48-60) or penetratin) conjugated to siRNA sense strand; duplex siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | L929 (mouse fibroblast) |
| Animal Model | |
| Administration Route | |
| Output Type | p38 MAPK mRNA knockdown (RT-qPCR, 24 h) |
| Output Value | ~36% knockdown at 10 µM (vs controls) |
| Output Units | |
| Output Notes | No effect for naked siRNA without transfection reagent; conjugate gives small but significant knockdown. |
| Toxicity Notes | No significant cellular toxicity reported at 24 h (MTT). |
| Curation Notes |