A Novel Cell-penetrating Peptide, M918, for Efficient Delivery of Proteins and Peptide Nucleic Acids (2007)
Sequence: AGYLLGK*INLKALAALAKKIL-NH2
| Experiment Id | EXP001780 |
|---|---|
| Paper | A Novel Cell-penetrating Peptide, M918, for Efficient Delivery of Proteins and Peptide Nucleic Acids |
| Peptide | Transportan 10 (TP10) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 5 µM (CPP-PNA conjugate) |
| Rna Concentration | 5 µM (PNA conjugate), 1 h treatment; readout after 16 h |
| Mixing Ratio | covalent disulfide conjugate (N/A) |
| Formulation Format | CPP–PNA covalent conjugate (disulfide-linked) |
| Formulation Components | TP10-PNA (antisense 705 PNA) disulfide-linked conjugate |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa pLuc 705 (splice correction luciferase reporter) |
| Animal Model | |
| Administration Route | |
| Output Type | Splice correction (luciferase activity) |
| Output Value | ~10-fold increase vs untreated at 5 µM TP10-PNA |
| Output Units | |
| Output Notes | Splice correction dose-dependent; chloroquine increases splice correction, indicating endosomal trapping limits bioavailability. |
| Toxicity Notes | TP10 used as positive control for membrane leakage; substantial LDH leakage at 25 µM reported. |
| Curation Notes |