Sequence: RQIKIWFQNRRMKWKKGGC
| Experiment Id | EXP001781 |
|---|---|
| Paper | Enhanced siRNA delivery into cells by exploiting the synergy between targeting ligands and cell-pene |
| Peptide | Penetratin (ANTP) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 100 nM siRNA (final in culture medium) |
| Mixing Ratio | siRNA-spermidine N/P 8:1 during encapsulation; DSPE-PEG conjugate:PLGA 1.6–25.9 mg/mg during fabrication |
| Formulation Format | PLGA nanoparticles surface-coated with DSPE-PEG-penetratin; siRNA-loaded (encapsulated) |
| Formulation Components | PLGA NP with DSPE-PEG-ANTP (penetratin) on surface; luciferase siRNA (siLUC) encapsulated via spermidine complexation; PEG-DSPE coating |
| Size Nm | 150.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | KB cells stably expressing luciferase (folate receptor positive; folate-free RPMI) |
| Animal Model | |
| Administration Route | |
| Output Type | Luciferase knockdown (48 h; normalized to total protein) |
| Output Value | ~30% knockdown vs untreated |
| Output Units | |
| Output Notes | Negative control siRNA in NP not significant vs untreated. |
| Toxicity Notes | Trypan blue used to quench extracellular fluorescence in uptake assay; no major toxicity focus reported. |
| Curation Notes |