Sequence: RQIKIWFQNRRMKWKKGGC
| Experiment Id | EXP001782 |
|---|---|
| Paper | Enhanced siRNA delivery into cells by exploiting the synergy between targeting ligands and cell-pene |
| Peptide | Penetratin (ANTP) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 100 nM siRNA (final in culture medium) |
| Mixing Ratio | siRNA-spermidine N/P 8:1 during encapsulation; ligands added as DSPE-PEG conjugates during 2nd emulsion |
| Formulation Format | Dual-ligand PLGA nanoparticles (folate + penetratin) via DSPE-PEG linkers; siRNA-loaded |
| Formulation Components | PLGA NP coated with DSPE-PEG-folate and DSPE-PEG-penetratin (ANTP/FOL-NP); luciferase siRNA encapsulated (spermidine complexation) |
| Size Nm | 150.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | KB luciferase cells |
| Animal Model | |
| Administration Route | |
| Output Type | Luciferase knockdown (48 h; normalized to total protein) |
| Output Value | ~63% knockdown vs untreated |
| Output Units | |
| Output Notes | Knockdown reduced to ANTP-NP level when excess free folate (2 mM) added, indicating folate receptor involvement; negative control siRNA NP not significant. |
| Toxicity Notes | No major toxicity focus reported. |
| Curation Notes |