Sequence: Fluo-LLIILRRRIRKQAHAHSK-amide
| Experiment Id | EXP001795 |
|---|---|
| Paper | Quantitatively determined uptake of cell-penetrating peptides in non-mammalian cells with an evaluat |
| Peptide | pVEC |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 0.1, 1.0, 10 µM (uptake assays; 1 h) |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | Free fluorescently-labeled CPP (no RNA cargo) |
| Formulation Components | Fluorescein-labeled CPP only; surface-bound peptide quenched/modified by diazotized 2-nitroaniline prior to lysis; uptake quantified by fl-HPLC |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Saccharomyces cerevisiae (yeast) |
| Animal Model | |
| Administration Route | |
| Output Type | CPP uptake (fl-HPLC; nmol/mg protein) and degradation profile |
| Output Value | At 10 µM: degraded MAP and penetratin ~30 nmol/mg protein; intact pVEC and all-D-pVEC ~10 nmol/mg; intact MAP/penetratin low at 0.1–1 µM and remain low even at 10 µM. |
| Output Units | |
| Output Notes | Incubation 1 h at 30°C; MAP/penetratin highly degraded; pVEC and all-D-pVEC largely intact. Antimicrobial activity (MIC): E. coli 25 µM for MAP/penetratin/pVEC; B. megaterium 1 µM for MAP/penetratin/pVEC; S. cerevisiae no inhibition at ≤25 µM. |
| Toxicity Notes | Antimicrobial activity (MIC): E. coli 25 µM for MAP/penetratin/pVEC; B. megaterium 1 µM for MAP/penetratin/pVEC; S. cerevisiae no inhibition at ≤25 µM. |
| Curation Notes |