Sequence: Stearyl-WLRLLWKKWFLKL-amide
| Experiment Id | EXP001815 |
|---|---|
| Paper | Magnetic Nanoparticle Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes |
| Peptide | PF222 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | CPP 10.5 µL used in formulation (per methods) |
| Rna Concentration | pGL3 (4 µL at 205 ng/µL; 10 µL complex added per well) |
| Mixing Ratio | CR 5:1 (CPP:pGL3) with MNPs co-incubated |
| Formulation Format | CPP/pDNA/MNP ternary nanocomplex (non-covalent magnetofection platform) |
| Formulation Components | PF222 + pGL3 + Fe3O4 magnetic nanoparticles (MNPs, 6.4 nm; 4 µL of 1 mg/mL stock) self-assembled in MQ water |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa cells (96-well luciferase expression assay, 24 h) |
| Animal Model | |
| Administration Route | |
| Output Type | Reporter expression (luciferase activity, RLU) |
| Output Value | Higher activity for several peptides vs CPP/pGL3 alone (notably PF221/PF222/PF223 reported as improved) |
| Output Units | |
| Output Notes | MNP incorporation increased pGL3 transfection for PF221/PF222/PF223 (reported 2–100× vs corresponding CPP/pGL3). |
| Toxicity Notes | WST-1 shows no significant toxicity for CPP/pGL3±MNPs after 24 h (supplementary). |
| Curation Notes |