Sequence: Stearyl-AGYLLGKLLOOLAAAALOOLL-NH2
| Experiment Id | EXP001820 |
|---|---|
| Paper | Magnetic Nanoparticle Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes |
| Peptide | PF14 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | CPP solution 10.5 µL used in formulation (per methods) |
| Rna Concentration | pGL3 (added as 4 µL at 205 ng/µL during complexation; 10 µL complex added per well) |
| Mixing Ratio | Charge ratio (CR) 5:1 (CPP:pGL3) |
| Formulation Format | CPP/pDNA nanocomplex (non-covalent) |
| Formulation Components | PF14 complexed with pGL3 in water; no magnetic nanoparticles |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa cells (96-well luciferase expression assay, 24 h) |
| Animal Model | |
| Administration Route | |
| Output Type | Reporter expression (luciferase activity, RLU) |
| Output Value | Baseline CPP/pGL3 activity; varies by peptide |
| Output Units | |
| Output Notes | Assayed at CR5; compared vs corresponding CPP/pGL3-MNPs and untreated. |
| Toxicity Notes | WST-1 shows no significant toxicity for CPP/pGL3±MNPs after 24 h (supplementary). |
| Curation Notes |