Sequence: Stearyl-AGYLLGKLLOOLAAAALOOLL-NH2
SCO (splice-correcting oligonucleotide / ASO)
| Experiment Id | EXP001832 |
|---|---|
| Paper | Magnetic Nanoparticle Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes |
| Peptide | PF14 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | CPP 10.5 µL used in formulation (per methods) |
| Rna Concentration | SCO 100 nM typical (varied 50–200 nM in optimization) |
| Mixing Ratio | Molar ratio (MR) 10:1 (CPP:SCO) |
| Formulation Format | CPP/SCO nanocomplex (non-covalent) |
| Formulation Components | PF14 complexed with SCO in MQ water; no MNPs |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa puLuc 705 cells (96-well splice correction, 24 h) |
| Animal Model | |
| Administration Route | |
| Output Type | Splice correction (luciferase activity) |
| Output Value | Splice correction activity varies by peptide; PF14 is benchmark |
| Output Units | |
| Output Notes | Measured at MR10; compared vs CPP/SCO-MNPs; PF14-SCO previously reported high vs Lipofectamine 2000. |
| Toxicity Notes | No significant toxicity reported (WST-1; supplementary). |
| Curation Notes |