Sequence: Stearyl-AGYLLGKLLOOLAAAALOOLL-NH2
| Experiment Id | EXP001835 |
|---|---|
| Paper | Magnetic Nanoparticle Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes |
| Peptide | PF14 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | PF14 added as 10.5 µL during complexation (per methods) |
| Rna Concentration | 25 nM and 50 nM tested |
| Mixing Ratio | MR 20:1 (PF14:siRNA) |
| Formulation Format | PF14/siRNA/MNP ternary nanocomplex (non-covalent) |
| Formulation Components | PF14 + luciferase siRNA (MR 20) + Fe3O4 MNPs (4 µL of 1 mg/mL stock) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U-87 MG-luc2 cells (96-well; 24 h knockdown via luminescence decrease) |
| Animal Model | |
| Administration Route | |
| Output Type | Gene silencing (luciferase knockdown; luminescence decrease) |
| Output Value | At 25 nM siRNA: similar with/without MNPs; at 50 nM: PF14-siRNA-MNPs stronger knockdown than PF14-siRNA |
| Output Units | |
| Output Notes | PF14 selected due to higher siRNA efficiency vs other CPPs; MNPs enhance at higher siRNA concentration. |
| Toxicity Notes | No significant toxicity reported for complexes; MNPs biocompatible (WST-1; supplementary). |
| Curation Notes |