Sequence: Stearyl-AGYLLGKLLOOLAAAALOOLL-NH2
| Experiment Id | EXP001836 |
|---|---|
| Paper | Magnetic Nanoparticle Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes |
| Peptide | PF14 |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | PF14 at CR4 (amount per dose not explicitly stated in text) |
| Rna Concentration | 20 µg pDNA per mouse (single dose); 150 µL MQ incubation then diluted to 200 µL in 5% glucose |
| Mixing Ratio | Charge ratio (CR) 4:1 (PF14:pDNA) |
| Formulation Format | PF14/pDNA nanocomplex (systemic) |
| Formulation Components | PF14 complexed with pDNA (CR4) in MQ; injected i.v. in 5% glucose |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Hsd:Athymic Nude-Foxn1nu female mice (4–6 weeks); n=3–5 |
| Administration Route | Intravenous (tail vein), single dose; tissues analyzed 24 h post injection |
| Output Type | In vivo reporter expression (luciferase RLU/mg protein in tissues vs naked pDNA) |
| Output Value | Transfection of lungs and spleen; PF14-pDNA-MNP comparable to PF14, but liver expression decreased (~18×) with MNPs reported |
| Output Units | |
| Output Notes | Tissue homogenates assayed post-mortem; results presented as fold increase over naked pDNA per tissue. |
| Toxicity Notes | No major acute toxicity described for single-dose study; animal study approved (Estonia). |
| Curation Notes |