Sequence: GRKKRRQRRRCG
| Experiment Id | EXP001842 |
|---|---|
| Paper | Development of an Efficient Transdermal Delivery System of Small Interfering RNA Using Functional Pe |
| Peptide | Tat analog |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | siRelA 0.05 mg per well (24-well) in 100 µL dosing volume |
| Mixing Ratio | N/P = 10 (Tat:siRNA) |
| Formulation Format | Tat/siRNA nanocomplex (electrostatic) |
| Formulation Components | Tat analog complexed with siRelA (incubated 30 min at 4°C); applied to PAM212 keratinocytes |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | PAM212 mouse keratinocytes |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional RNA effect (RelA mRNA silencing by qRT-PCR) |
| Output Value | Significant RelA mRNA knockdown with Tat/siRelA at N/P 10 vs naked siRelA |
| Output Units | |
| Output Notes | Cells transfected 24 h then stimulated with LPS for 8 h prior to RT-PCR readout. |
| Toxicity Notes | |
| Curation Notes |