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EXP001844

Paper

Development of an Efficient Transdermal Delivery System of Small Interfering RNA Using Functional Peptides, Tat and AT-1002 (2011)

Peptide

Tat analog + AT1002 analog (AT-1002)

Sequence: GRKKRRQRRRCG | FCIGRLCG

RNA

siRNA

All experiment fields

Experiment Id EXP001844
Paper Development of an Efficient Transdermal Delivery System of Small Interfering RNA Using Functional Pe
Peptide Tat analog + AT1002 analog (AT-1002)
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect no
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration
Mixing Ratio Tat/siRNA at N/P 10, then AT1002 added (AT1002:siRNA weight ratio 80:1)
Formulation Format Tat/siRNA nanocomplex + AT1002 co-formulation
Formulation Components Tat/siRelA complex incubated then mixed with AT1002; evaluated for RNaseA protection
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells
Animal Model
Administration Route
Output Type siRNA stability against RNaseA
Output Value ~60% siRNA remaining after 10 h RNaseA (strongest protection among conditions)
Output Units
Output Notes Demonstrates synergistic stabilization of siRNA by Tat + AT1002.
Toxicity Notes
Curation Notes