Sequence: GRKKRRQRRRCG
| Experiment Id | EXP001845 |
|---|---|
| Paper | Development of an Efficient Transdermal Delivery System of Small Interfering RNA Using Functional Pe |
| Peptide | Tat analog |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | Tat 32 µg with 5 µg siRNA (reported for topical dosing) |
| Rna Concentration | 5 µg fluorescent siRNA per application (25 µL); 10 h |
| Mixing Ratio | Tat/siRNA N/P = 10; topical application |
| Formulation Format | Topical Tat/siRNA complexes for transdermal delivery (tape-stripped skin) |
| Formulation Components | Tat/FAMsiRNA or Tat/Cy3-siGL3 applied to mouse back skin (25 µL) after tape stripping |
| Size Nm | 70.00 |
| Zeta Mv | 4.00 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | ICR mice (male, 6-week); back skin tape-stripped (0/10/20 times); topical 10 h |
| Administration Route | Topical application to tape-stripped dorsal skin (25 µL); imaging on frozen sections by confocal microscopy |
| Output Type | In vivo uptake/distribution in skin (confocal microscopy) |
| Output Value | With 20× tape stripping, Tat increases siRNA localization mainly in hair follicles and into epidermal/dermal layers vs naked |
| Output Units | |
| Output Notes | Tat alone shows follicular localization; deeper signal reduced beyond ~10 µm depth vs AT1002. |
| Toxicity Notes | |
| Curation Notes |