Sequence: RXRRXRRXRRXRXB
| Experiment Id | EXP001851 |
|---|---|
| Paper | Impact of different cell penetrating peptides on the efficacy of antisense therapeutics for targetin |
| Peptide | PRXR |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | Fixed covalent conjugate (CPP linked to PNA via -O- linker) |
| Formulation Format | Covalent CPP–PNA conjugate (antisense construct) |
| Formulation Components | RXRRXRRXRRXRXB-O-cgatcattcaaa-NH2 |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | J774A.1 murine macrophages infected with L. monocytogenes F4244 (MOI 10:1); gentamicin protection assay |
| Animal Model | |
| Administration Route | |
| Output Type | Intracellular antibacterial efficacy (log CFU reduction of intracellular Listeria after 4 h treatment) |
| Output Value | At 2 µM: ~1.78 log reduction; 4 µM: ~3.65; 8 µM: complete clearance (reported 6.73 log reduction). |
| Output Units | |
| Output Notes | CPP–PNA constructs added at final 2, 4, 8 µM; intracellular bacteria enumerated by plating after macrophage lysis. |
| Toxicity Notes | |
| Curation Notes |