Sequence: RQIKIWFQNRRMKWKK
| Experiment Id | EXP001854 |
|---|---|
| Paper | Impact of different cell penetrating peptides on the efficacy of antisense therapeutics for targetin |
| Peptide | PANT |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | CPP–PNA concentration 16 or 32 µM |
| Rna Concentration | CPP–PNA concentration 16 or 32 µM |
| Mixing Ratio | Fixed covalent conjugate (CPP linked to PNA via -O- linker) |
| Formulation Format | Covalent CPP–PNA conjugate (antisense construct) |
| Formulation Components | RQIKIWFQNRRMKWKK-O-cgatcattcaaa-NH2 |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | C. elegans AU37 [sek-1(km4); glp-4(bn2)] infected with L. monocytogenes J0161 (18 h treatment) |
| Administration Route | Worm incubation in low-binding tubes with CPP–PNA at 16 or 32 µM after infection; CFU counted from lysed worms |
| Output Type | In vivo efficacy in infection model (bacterial load reduction/clearance in C. elegans) |
| Output Value | Significant reduction only at 32 µM (~1.89 log CFU reduction). |
| Output Units | |
| Output Notes | Demonstrates antisense therapeutic efficacy in a live-animal infection model (nematode). |
| Toxicity Notes | |
| Curation Notes |