Sequence: Ac-GLWRALWRLLRSLWRLLWRA-cya
| Experiment Id | EXP001864 |
|---|---|
| Paper | Insight into the Cellular Uptake Mechanism of a Secondary Amphipathic Cell-Penetrating Peptide for s |
| Peptide | CADY |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | CADY:siRNA molar ratios studied 10:1–80:1 (complex formation 30 min at 37°C; key structural transition optimal around 40:1 in CD) |
| Formulation Format | Noncovalent CADY/siRNA nanoparticle complex |
| Formulation Components | CADY peptide (1 mg/mL stock) incubated with siRNA to form stable complexes; studied with lipid vesicles (DOPC/DOPG etc.) and heparan sulfate proteoglycan competition |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | |
| Animal Model | |
| Administration Route | |
| Output Type | Mechanistic/biophysical characterization of CADY/siRNA membrane interaction (CD, fluorescence, monolayer insertion, gel shift with HSPG) |
| Output Value | CADY binds siRNA tightly (Kd ~47 nM) and undergoes random-coil→α-helix transition; CADY/siRNA interacts strongly with phospholipids; uptake proposed to be endosomal-pathway independent |
| Output Units | |
| Output Notes | This paper focuses on uptake mechanism and membrane interaction; it does not present in vitro gene knockdown or in vivo efficacy endpoints. |
| Toxicity Notes | |
| Curation Notes |