Penetratin — NPs-3 (HA 200 kDa, high density shell)
Sequence: RQIKIWFQNRRMKWKK
| Experiment Id | EXP001882 |
|---|---|
| Paper | Fine Tuning of Core–Shell Structure of Hyaluronic Acid/Cell-Penetrating Peptides/siRNA Nanoparticles |
| Peptide | Penetratin — NPs-3 (HA 200 kDa, high density shell) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | CPP/siRNA N/P = 4:1 (complete complexation); HA coated by adsorption to produce NPs-1/NPs-2/NPs-3. |
| Formulation Format | Core–shell nanoparticle (Penetratin/siRNA core; hyaluronic acid shell for NPs) |
| Formulation Components | Core: penetratin-condensed LOX-1 siRNA (NCs). Shell: HA 200 kDa, high density shell; HAase-responsive de-shielding in plaques/macrophage environment. |
| Size Nm | 153.10 |
| Zeta Mv | 30.50 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HUVEC injured endothelium (TNF-α stimulated) – THP-1-derived macrophage coculture (transwell); uptake by microscopy + flow cytometry; LOX-1 mRNA knockdown by qRT-PCR at 48 h; foam cell assays (Oil Red O, DiI-oxLDL). |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional RNA effect: LOX-1 mRNA knockdown (qRT-PCR) + reduced foam-cell lipid accumulation |
| Output Value | LOX-1 mRNA reduced vs control; gene-silencing and anti-foam-cell effects increased in order NCs < NPs-1 < NPs-2 < NPs-3 (best). |
| Output Units | |
| Output Notes | HA-coated NCs using 200 kDa HA with higher coating density; best-performing formulation. Uptake of Cy3-siRNA formulations quantified by flow cytometry and fluorescence microscopy; uptake inhibited by free HA pre-blocking (CD44-mediated). |
| Toxicity Notes | |
| Curation Notes |