Rational Design of a Biomimetic Cell Penetrating Peptide Library (2013)
Sequence: SPDEREEWMRAIQMVANSLK
| Experiment Id | EXP001936 |
|---|---|
| Paper | Rational Design of a Biomimetic Cell Penetrating Peptide Library |
| Peptide | Peptide 50 (RAC-beta) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | Nanoparticles: total lipids:siRNA weight ratio 10:1; peptide added post-formation at ~5-fold molar excess relative to PEG-thiol. |
| Formulation Format | Peptide-decorated lipidoid nanoparticle (C12-117 LNP) carrying siRNA |
| Formulation Components | Lipidoid nanoparticle (C12-117) + cholesterol + DSPE-PEG-SH (PEG2k/3.4k) encapsulating/adsorbing siRNA; peptides synthesized with N-term maleimide-PEG8 and conjugated post-formation to surface thiols. |
| Size Nm | 96.30 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Dual-luciferase HeLa cells (firefly + renilla reporters) |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional RNA effect: luciferase knockdown (Dual-Glo assay at day 3) |
| Output Value | Luciferase silencing measured; activity varies across peptide-decorated nanoparticles (top hits reached ~60–95% knockdown at 2.5 µg/mL siRNA). |
| Output Units | |
| Output Notes | Cells treated 4 h then media replaced; readout at 3 days. C12-117 LNP without peptide shows no silencing. Stoichiometric 1:1 peptide–siRNA conjugates had only modest (~≤30%) knockdown. |
| Toxicity Notes | |
| Curation Notes |