Rational Design of a Biomimetic Cell Penetrating Peptide Library (2013)
Sequence: CRRWQWRMKKLGC
| Experiment Id | EXP001940 |
|---|---|
| Paper | Rational Design of a Biomimetic Cell Penetrating Peptide Library |
| Peptide | Lactoferricin (short, 13 aa) |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | Total lipids:siRNA weight ratio 10:1; peptide added post-formation (maleimide to surface thiols). |
| Formulation Format | Peptide-decorated lipidoid nanoparticle (C12-117 LNP) carrying siRNA |
| Formulation Components | Lipidoid nanoparticle (C12-117) + cholesterol + DSPE-PEG-SH (PEG2k/3.4k) encapsulating/adsorbing siRNA; peptides synthesized with N-term maleimide-PEG8 and conjugated post-formation to surface thiols. |
| Size Nm | 90.00 |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | C57BL/6 mice |
| Administration Route | Tail vein i.v. injection; 1 mg/kg and 5 mg/kg siRNA; serum collected day 3 for FVII ELISA |
| Output Type | In vivo functional delivery: serum Factor VII reduction (chromogenic ELISA) |
| Output Value | Significant in vivo knockdown of circulating Factor VII. Reported approximate efficacy: lactoferricin highest (~80–90% at 5 mg/kg), orexin ~70–80%, surfactant protein B ~50%. |
| Output Units | |
| Output Notes | Nanoparticles primarily accumulate in liver/lung by Cy5.5-siRNA imaging and micro-CT with thyroxine-siRNA contrast agent; efficacy correlates with biodistribution. |
| Toxicity Notes | |
| Curation Notes |