Sequence: GALA (fusogenic CPP) — G-NP
| Experiment Id | EXP001946 |
|---|---|
| Paper | Co-delivery of EGFR and BRD4 siRNA by cell-penetrating peptides-modified redox-responsive complex in |
| Peptide | GALA (fusogenic CPP) — G-NP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | N/P = 30 (optimal) |
| Formulation Format | Electrostatic polyplex (PEG-SS-PEI/siRNA) with CPP (GALA and/or CREKA) modification |
| Formulation Components | G-NP: GALA-PEG-SS-PEI complexed with siRNA (siEGFR:siBRD4 = 1:1) |
| Size Nm | 70.00 |
| Zeta Mv | 8.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MDA-MB-231 (TNBC) for uptake/gene silencing/phenotype; HUVEC for biocompatibility |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional RNA effect: gene expression (qRT-PCR/western) + invasion/migration assays |
| Output Value | Improved transfection vs non-CPP polymer; gene silencing and reduced invasion/migration vs control |
| Output Units | |
| Output Notes | Cellular uptake shown by confocal + flow cytometry (FAM-siRNA). Endosomal escape specifically assayed for GC-NP; for G-NP defaulted to NaN. |
| Toxicity Notes | |
| Curation Notes |