Sequence: RLWRLLWRLWRRLRWRLLR
| Experiment Id | EXP001950 |
|---|---|
| Paper | Serum Stability and Physicochemical Characterization of a Novel Amphipathic Peptide C6M1 for SiRNA D |
| Peptide | C6M1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Peptide stock 1 mM; complexes formed at molar ratio (C6M1:siRNA) 30:1 for knockdown; incubated 20 min RT before use. |
| Rna Concentration | 50 nM siRNA final concentration (western blot knockdown); also tested 5–100 nM (optimum 40–50 nM). |
| Mixing Ratio | Molar ratio (C6M1:siRNA) = 30:1 for transfection (gel shift shows full encapsulation at ≥15:1; higher ratios improve stability). |
| Formulation Format | Noncovalent peptide/siRNA complex (electrostatic co-assembly) |
| Formulation Components | C6M1 amphipathic peptide complexed with GAPDH siRNA; complexes prepared in water then introduced to PBS and diluted into Opti-MEM for cell treatment. |
| Size Nm | 70.00 |
| Zeta Mv | 30.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | CHO-K1 (Chinese hamster ovary) cells |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional RNA effect: GAPDH protein knockdown (western blot) |
| Output Value | ≈72% decrease in GAPDH protein vs untreated (ImageJ quantification) at 50 nM siRNA, MR 30:1; naked siRNA and C6M1+NC-siRNA showed no significant knockdown |
| Output Units | |
| Output Notes | Study focuses on physicochemical characterization (DLS/TEM/CD/serum stability) and demonstrates in vitro functional knockdown in CHO-K1; no in vivo experiments. |
| Toxicity Notes | |
| Curation Notes |