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EXP001957

Paper

Identification of a novel cell-penetrating peptide targeting human glioblastoma cell lines as a cancer-homing transporter (2015)

Peptide

Peptide1NSD–p16 MIS (CPP–cargo fusion)

Sequence: TCTWLKYH-GPG-FLDTLVVLHR-GP-RRRR

RNA

All experiment fields

Experiment Id EXP001957
Paper Identification of a novel cell-penetrating peptide targeting human glioblastoma cell lines as a canc
Peptide Peptide1NSD–p16 MIS (CPP–cargo fusion)
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect no
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration
Mixing Ratio
Formulation Format Peptide alone or peptide–cargo fusion (no RNA payload)
Formulation Components FITC-labeled peptides for uptake assays; p16 MIS fusion peptides for functional cargo delivery (apoptosis/pRB phosphorylation).
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells U87MG and U118MG (glioblastoma); other lines for selectivity panel (HeLa, HepG2, A549, PANC-1, etc.)
Animal Model
Administration Route
Output Type Functional cargo delivery (apoptosis induction; pRB phosphorylation)
Output Value Early apoptosis increased to ~70% (Annexin V+/PI−) vs ~10–12% controls; phosphorylated pRB (Ser807/811) decreased vs controls.
Output Units
Output Notes Demonstrates CPP-mediated intracellular delivery of a functional peptide cargo (not RNA).
Toxicity Notes
Curation Notes