Sequence: RRRRRRRR
| Experiment Id | EXP001992 |
|---|---|
| Paper | Cell-Penetrating Peptide-Modified Graphene Oxide Nanoparticles Loaded with Rictor siRNA for the Trea |
| Peptide | Octaarginine (R8) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | CPP solution added to GO-PEI-PEG at final 1 mM during synthesis (then washed); transfection doses reported as siRNA 0–150 nM in assays. |
| Rna Concentration | Typically 50–150 nM siRNA in vitro (MTT uses 0, 50, 100, 150 nM). |
| Mixing Ratio | Optimum mass ratio siRNA : GO-PEI-PEG-CPP = 1 : 0.5 (gel retardation screen; GO:PEI ratio 1:60–1:100 gives complete binding at 1:0.5). |
| Formulation Format | GO-PEI-PEG-CPP/siRNA nanocomplex (graphene oxide + PEI + PEG + CPP) |
| Formulation Components | Graphene oxide (GO) + polyethyleneimine (PEI, MW 800) + polyethylene glycol (PEG, MW 2000) + CPP R8, loaded with Rictor siRNA-2 (or Cy5-labeled siRNA for uptake). |
| Size Nm | 231.60 |
| Zeta Mv | 16.10 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MDA-MB-231 (triple-negative breast cancer) cells |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional RNA effect: Rictor knockdown (qPCR/WB) + phenotypic assays (MTT, colony formation, apoptosis) |
| Output Value | Rictor siRNA-2 reduces Rictor expression; GO-PEI-PEG-CPP/siRNA enhances uptake vs non-CPP and reduces viability / colonies and increases apoptosis vs controls. |
| Output Units | |
| Output Notes | Uptake shown by confocal microscopy and flow cytometry with Cy5-siRNA; functional effects include reduced Rictor and downstream p-Akt/p-p70s6k plus increased Annexin V/PI apoptosis. |
| Toxicity Notes | Body-weight effects addressed in vivo; in vitro cytotoxicity assessed by MTT/colony/apoptosis. |
| Curation Notes |