OK-102 (stearoyl-oligopeptide)
Sequence: CHHRRRRHHC
nkRNA1 (long ssRNA that self-anneals)
| Experiment Id | EXP001994 |
|---|---|
| Paper | Functional peptide nanocarriers for delivery of novel anti-RelA RNA interference agents as a topical |
| Peptide | OK-102 (stearoyl-oligopeptide) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Defined by N/P=10 complexation (OK-102 amount not explicitly stated). |
| Rna Concentration | 50 nM RNAi agent in transfection mixture; 24 h transfection then 8 h LPS stimulation. |
| Mixing Ratio | OK-102 complexes prepared at N/P (nitrogen:phosphorous) ratio = 10; mixed 30 min at room temperature before use. |
| Formulation Format | CPP peptide/RNA electrostatic complex (cytoplasm-responsive nanocarrier with disulfide crosslinking) |
| Formulation Components | OK-102 (stearoyl-CHHRRRRHHC) complexed with anti-RelA nkRNA1 or PnkRNA1. |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | RAW264.7 murine macrophage cells (LPS-stimulated after transfection) |
| Animal Model | |
| Administration Route | |
| Output Type | In vitro functional RNA effect: RelA mRNA silencing (qPCR) after LPS stimulation |
| Output Value | Significant reduction of RelA mRNA vs LPS-only control (sequence-specific; scrambled RNAi did not silence). |
| Output Units | |
| Output Notes | Demonstrates functional RNAi activity in macrophages using OK-102 complexes. |
| Toxicity Notes | |
| Curation Notes |