Sequence: PCM: CWLSEAGPVVTVRALRGTGSW; Tat: YGRKKRRQRRR
| Experiment Id | EXP002020 |
|---|---|
| Paper | Cell Targeting Peptide Conjugation to siRNA Polyplexes for Effective Gene Silencing in Cardiomyocyte |
| Peptide | PCM + Tat |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | 20 µg total nucleic acid per mouse |
| Rna Concentration | 20 µg total nucleic acid |
| Mixing Ratio | siRNA : pDNA = 1:1 |
| Formulation Format | siRNA-pDNA/CD polyplex |
| Formulation Components | PCM-siRNA + Tat-siRNA + plasmid DNA + poly(CBA-DAH) |
| Size Nm | 150.00 |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | BALB/c mice |
| Administration Route | intravenous (tail vein) |
| Output Type | biodistribution |
| Output Value | Highest accumulation in heart vs other organs |
| Output Units | |
| Output Notes | qPCR detection of reporter gene; no functional knockdown or phenotype measured |
| Toxicity Notes | No immune activation reported |
| Curation Notes |