Sequence: YGRKKRRQRRR
| Experiment Id | EXP002023 |
|---|---|
| Paper | Establishment of MicroRNA Delivery System by PP7 Bacteriophage-Like Particles Carrying Cell-Penetrat |
| Peptide | TAT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 10–100 nM (VLP concentration) |
| Rna Concentration | 10–100 nM (encapsidated pre-miRNA) |
| Mixing Ratio | |
| Formulation Format | virus-like particle (PP7 VLP) |
| Formulation Components | PP7 capsid + surface-displayed TAT peptide + packaged pre-miR-23b |
| Size Nm | 30.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | SK-HEP-1 hepatoma cells; Cos-7 cells |
| Animal Model | |
| Administration Route | |
| Output Type | miRNA expression, gene silencing, migration assay |
| Output Value | Increased mature miR-23b; CDH17 downregulation; reduced cell migration |
| Output Units | |
| Output Notes | miR-23b processed intracellularly; luciferase reporter and Western blot confirm functionality |
| Toxicity Notes | Low cytotoxicity; cell viability >90% up to 500 nM |
| Curation Notes |