Identification of a Novel Cell-Penetrating Peptide from Human Phosphatidate Phosphatase LPIN3 (2012)
Sequence: RRKRRRRRK
| Experiment Id | EXP002024 |
|---|---|
| Paper | Identification of a Novel Cell-Penetrating Peptide from Human Phosphatidate Phosphatase LPIN3 |
| Peptide | LPIN (LPIN3-derived CPP) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | 1–20 µM LPIN-EGFP |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | CPP–protein fusion |
| Formulation Components | LPIN peptide + EGFP |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Jurkat T cells; HeLa cells; primary mouse splenocytes |
| Animal Model | |
| Administration Route | |
| Output Type | cellular uptake / localization |
| Output Value | Efficient intracellular and nuclear uptake vs EGFP control |
| Output Units | |
| Output Notes | Uptake quantified by FACS; localization by confocal microscopy; lipid raft-mediated endocytosis |
| Toxicity Notes | No cytotoxicity reported |
| Curation Notes |