Cell-Penetrating Peptides Derived from Viral Capsid Proteins (2011)
Sequence: STSGTGKMTRAQRRRAAARRNR
| Experiment Id | EXP002029 |
|---|---|
| Paper | Cell-Penetrating Peptides Derived from Viral Capsid Proteins |
| Peptide | CPNT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 5–20 µM |
| Rna Concentration | 1 µg dsRNA (where applicable) |
| Mixing Ratio | ≈25:1 peptide:RNA (molar) |
| Formulation Format | free peptide ± RNA (noncovalent) |
| Formulation Components | CPNT peptide ± RNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Arabidopsis roots; barley protoplasts; barley mesophyll cells |
| Animal Model | |
| Administration Route | |
| Output Type | uptake / RNA delivery / gene silencing |
| Output Value | RNA delivery and functional gene silencing |
| Output Units | |
| Output Notes | Delivered viral RNA leading to replication and dsRNA causing ~50% PDS knockdown |
| Toxicity Notes | No toxicity observed in seedlings |
| Curation Notes |