Sequence: YGRKKRRQRRR
| Experiment Id | EXP002034 |
|---|---|
| Paper | Highly penetrating nanobubble polymer enhances LINC00511-siRNA delivery for improving the chemosensi |
| Peptide | TAT (CPP) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | CPP stock 1 mg/mL used for CNB preparation; (final per well not explicitly stated) |
| Rna Concentration | 100 pmol/well (6-well plate) for transfection; siRNA stock 20 μM |
| Mixing Ratio | CPP (1 mg/mL) mixed with siRNA (20 μM) during CNB preparation (ratio not specified) |
| Formulation Format | CPP-loaded nanobubbles + ultrasound-mediated destruction (UMND) |
| Formulation Components | DSPC:DSPE-PEG2000 (9:1) lipid shell + C3F8 gas + Rhodamine-B–TAT CPP + siLINC00511 |
| Size Nm | 366.50 |
| Zeta Mv | 7.97 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MDA-MB-231 (triple-negative breast cancer cells) |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown + chemosensitivity (IC50 shift) |
| Output Value | LINC00511 downregulated; cisplatin IC50 reduced from 13.01±1.36 to 9.78±1.01 (μg/mL) |
| Output Units | |
| Output Notes | CNBs-siLINC00511 + US showed highest transfection (~86.7% FAM+ by flow cytometry) and increased apoptosis with cisplatin; ultrasound: 1 MHz, 1 kHz PRF, 50% duty, 1.0 W/cm2, 30 s; nanobubbles 3×10^8/mL. |
| Toxicity Notes | Very low toxicity at 24 h post-transfection; proliferation inhibited at 48 h after transfection (reported). |
| Curation Notes |