3TD peptide (TAT–TAT–TAT–DRBD)
Sequence: YGRKKRRQRRR ×3 + DRBD
| Experiment Id | EXP002057 |
|---|---|
| Paper | Loading of cocktail siRNAs into extracellular vesicles via TAT-DRBD peptide for the treatment of cas |
| Peptide | 3TD peptide (TAT–TAT–TAT–DRBD) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | 4:1 (3TD:siRNA theoretical binding) |
| Formulation Format | Engineered extracellular vesicles (EVs) |
| Formulation Components | WPMY-1-derived EVs loaded via 3TD peptide |
| Size Nm | 144.90 |
| Zeta Mv | -18.40 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | LNCaP-AI (androgen-independent prostate cancer) |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown, apoptosis induction |
| Output Value | qRT-PCR knockdown of 3 genes; AR protein ↓76%; apoptosis ↑ |
| Output Units | |
| Output Notes | Robust multi-gene silencing without reported cytotoxicity |
| Toxicity Notes | No significant toxicity reported |
| Curation Notes |