siRNA suppression of hTERT using activatable cell-penetrating peptides in hepatoma cells (2015)
Sequence: EEEEEEE-GALGLP-RRRRRRRRKKR
| Experiment Id | EXP002058 |
|---|---|
| Paper | siRNA suppression of hTERT using activatable cell-penetrating peptides in hepatoma cells |
| Peptide | MMP2-activatable CPP (aCPP) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 2 μg plasmid per well (6-well) |
| Mixing Ratio | 30:1 (CPP:plasmid DNA) used in assays |
| Formulation Format | CPP–plasmid complex |
| Formulation Components | aCPP complexed with siRNA-encoding plasmid DNA (pRNAT-U6.1/Neo) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | SMMC-7721 hepatoma cells |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown + proliferation inhibition |
| Output Value | hTERT mRNA ↓26%; proliferation inhibition ~34.8%; G1 arrest |
| Output Units | |
| Output Notes | Functional RNA interference shown via qRT-PCR and phenotypic assays |
| Toxicity Notes | Reported low cytotoxicity (non-viral delivery) |
| Curation Notes |