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EXP002064

Paper

Encapsulation of mRNA into Artificial Viral Capsids via Hybridization of a β-Annulus-dT20 Conjugate and the Poly(A) Tail of mRNA (2020)

Peptide

Cys-β-annulus (dT20-SS)

Sequence: CINHVGGTGGAIMAPVAVTRQLVGS

RNA

mRNA

All experiment fields

Experiment Id EXP002064
Paper Encapsulation of mRNA into Artificial Viral Capsids via Hybridization of a β-Annulus-dT20 Conjugate
Peptide Cys-β-annulus (dT20-SS)
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration 50 µM (in vitro)
Rna Concentration 1 µM
Mixing Ratio dT20-SS-β-annulus : β-annulus = 1:9
Formulation Format Artificial viral capsid
Formulation Components dT20-SS-β-annulus + β-annulus co-assembly
Size Nm 52.00
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells HepG2 cells
Animal Model
Administration Route
Output Type reporter expression
Output Value Robust mCherry expression only after His16 surface modification
Output Units
Output Notes Functional mRNA expression enabled by CPP (His16)
Toxicity Notes No toxicity reported
Curation Notes