Sequence: GALFLGFLGAAGSTMGA-WSQ-PKSKRKV
| Experiment Id | EXP002065 |
|---|---|
| Paper | Efficient delivery of miR-122 to regulate cholesterol metabolism using a non-covalent peptide-based |
| Peptide | MPGΔNLS |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 1 mM |
| Rna Concentration | 50 nM |
| Mixing Ratio | 20:1 (peptide:miRNA) |
| Formulation Format | Non-covalent peptide/miRNA complex |
| Formulation Components | MPGΔNLS + miR-122 mimic or inhibitor |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | NCTC 1469, A549, mouse primary hepatocytes |
| Animal Model | |
| Administration Route | |
| Output Type | gene regulation / metabolic effect |
| Output Value | miR-122 up/downregulated; cholesterol levels altered |
| Output Units | |
| Output Notes | Robust functional miRNA activity without cytotoxicity |
| Toxicity Notes | No significant toxicity up to 100 nM |
| Curation Notes |