Sequence: Ac-YIGSR-AVA-LWRALWRLLRSLWRLLWKR-NH2 + Ac-PEG2-LWRALWRLLRSLWRLLWKR-NH2
CRISPR RNA (Cas9 mRNA + sgRNA)
| Experiment Id | EXP002070 |
|---|---|
| Paper | Enhanced CRISPR-Cas9 RNA system delivery using cell penetrating peptides-based nanoparticles for eff |
| Peptide | ADGN (AVA+PEG) |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 0.5 mg/kg RNA |
| Mixing Ratio | R=200 |
| Formulation Format | Peptide-based nanoparticle |
| Formulation Components | ADGN-AVA + ADGN-PEG co-assembled with RNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Orthotopic A549-luc lung tumor in mice |
| Administration Route | intravenous |
| Output Type | in vivo gene knockout |
| Output Value | >50% reduction in bioluminescence signal |
| Output Units | |
| Output Notes | Functional in vivo CRISPR delivery |
| Toxicity Notes | No liver/kidney toxicity |
| Curation Notes |