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EXP002072

Paper

Surface-Engineered Mucus Penetrating Nucleic Acid Delivery Systems with Cell Penetrating Peptides for the Lungs (2022)

Peptide

M9 (CS-A–mannitol coated)

Sequence: CRRLRHLRHHYRRRWHRFRC

RNA

plasmid DNA / siRNA

All experiment fields

Experiment Id EXP002072
Paper Surface-Engineered Mucus Penetrating Nucleic Acid Delivery Systems with Cell Penetrating Peptides fo
Peptide M9 (CS-A–mannitol coated)
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration 40 ng/µL DNA; 20 nM siRNA
Mixing Ratio N/P = 10
Formulation Format Peptide–RNA nanocomplex with polymer coating
Formulation Components M9 + nucleic acid core; CS-A–mannitol coating
Size Nm 250.00
Zeta Mv 20.00
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells A549, BEAS-2B
Animal Model
Administration Route
Output Type reporter expression
Output Value Highest GFP expression among tested systems
Output Units
Output Notes Superior mucus penetration and cellular transfection
Toxicity Notes Low cytotoxicity
Curation Notes