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EXP002073

Paper

Surface-Engineered Mucus Penetrating Nucleic Acid Delivery Systems with Cell Penetrating Peptides for the Lungs (2022)

Peptide

M9 (CS-A–mannitol coated)

Sequence: CRRLRHLRHHYRRRWHRFRC

RNA

plasmid DNA

All experiment fields

Experiment Id EXP002073
Paper Surface-Engineered Mucus Penetrating Nucleic Acid Delivery Systems with Cell Penetrating Peptides fo
Peptide M9 (CS-A–mannitol coated)
Delivery Success Class no
In Vivo Flag yes
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration 8 µg DNA per mouse
Mixing Ratio N/P = 10
Formulation Format Peptide–DNA nanocomplex with polymer coating
Formulation Components M9 + plasmid DNA; CS-A–mannitol coating
Size Nm
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model C57BL/6 mouse lung
Administration Route intratracheal
Output Type biodistribution
Output Value Widespread lung parenchyma distribution
Output Units
Output Notes Same M9 (CS-A–mannitol coated) formulation as in vitro studies; in vivo experiment assessed biodistribution only, not gene expression
Toxicity Notes No acute toxicity reported
Curation Notes