Sequence: GRKKRRQRRRPPQC-amide
| Experiment Id | EXP002074 |
|---|---|
| Paper | Macropinocytosis activated by oncogenic Dbl enables specific targeted delivery of Tat/pDNA nano-comp |
| Peptide | HIV-Tat |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | Varied (N/P 1–20) |
| Rna Concentration | 10 µg pDNA per transfection |
| Mixing Ratio | N/P = 1–20 |
| Formulation Format | Tat/pDNA nanocomplex |
| Formulation Components | Tat peptide + pGL3 plasmid DNA |
| Size Nm | 600.00 |
| Zeta Mv | 8.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | SKOV3, Cos-7, HeLa, HepG2, 7721, 293, 3T3 |
| Animal Model | |
| Administration Route | |
| Output Type | reporter gene expression |
| Output Value | High luciferase expression selectively in SKOV3 ovarian cancer cells |
| Output Units | |
| Output Notes | Cell-type-specific functional gene expression via macropinocytosis |
| Toxicity Notes | No significant cytotoxicity reported |
| Curation Notes |