Design and Evaluation of Endosomolytic Biocompatible Peptides as Carriers for siRNA Delivery (2015)
Sequence: HLLRLLLRLWHRLLRLLR
| Experiment Id | EXP002081 |
|---|---|
| Paper | Design and Evaluation of Endosomolytic Biocompatible Peptides as Carriers for siRNA Delivery |
| Peptide | C6M4 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 50–100 nM |
| Mixing Ratio | 20:1–60:1 |
| Formulation Format | noncovalent peptide/siRNA complex |
| Formulation Components | peptide + siRNA |
| Size Nm | 100.00 |
| Zeta Mv | 30.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | CHO-K1 |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | Detectable GAPDH knockdown in screening assay; weaker than C6M3/C6M10 |
| Output Units | |
| Output Notes | All peptides screened; strongest: C6M3, C6M6 |
| Toxicity Notes | Low toxicity |
| Curation Notes |