A Novel p19 Fusion Protein as a Delivery Agent for Short-interfering RNAs (2016)
Sequence: Linked p19 dimer fused to TAT (RKKRRQRRR)
| Experiment Id | EXP002101 |
|---|---|
| Paper | A Novel p19 Fusion Protein as a Delivery Agent for Short-interfering RNAs |
| Peptide | 2x-p19-TAT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | no |
| Peptide Concentration | 0–200 nmol/L |
| Rna Concentration | 500 nmol/L |
| Mixing Ratio | 10:1 (protein:siRNA) |
| Formulation Format | protein–siRNA complex |
| Formulation Components | 2x-p19-TAT + siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Huh7 |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | Potent and sustained luciferase knockdown (~96% at 72 h) |
| Output Units | |
| Output Notes | TAT mediates uptake; p19 binds siRNA with high affinity; endosomal entrapment limits efficiency |
| Toxicity Notes | Non-cytotoxic vs RNAiMAX |
| Curation Notes |