PepFect14 mediates the delivery of mRNA into human primary keratinocytes and in vivo (2023)
Sequence: Stearyl-AGYLLGKLLOOLAAAALOOLL-NH2
| Experiment Id | EXP002107 |
|---|---|
| Paper | PepFect14 mediates the delivery of mRNA into human primary keratinocytes and in vivo |
| Peptide | PepFect14 (PF14) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | ~1 µM (CR 2:1) |
| Rna Concentration | ~0.8 ng/µL |
| Mixing Ratio | Charge ratio 2:1 (PF14:mRNA) |
| Formulation Format | noncovalent peptide–mRNA nanoparticles |
| Formulation Components | PF14 ± CaCl2 / PS80 / MgCl2 |
| Size Nm | 100.00 |
| Zeta Mv | 25.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa, HaCaT, primary human keratinocytes |
| Animal Model | |
| Administration Route | |
| Output Type | reporter expression |
| Output Value | Robust reporter expression (additive-dependent) |
| Output Units | |
| Output Notes | CaCl2 enhances in HeLa/HaCaT; PS80 enhances in keratinocytes |
| Toxicity Notes | Generally low; PS80 reduces keratinocyte viability |
| Curation Notes |