PepFect14 mediates the delivery of mRNA into human primary keratinocytes and in vivo (2023)
Sequence: Stearyl-AGYLLGKLLOOLAAAALOOLL-NH2
| Experiment Id | EXP002110 |
|---|---|
| Paper | PepFect14 mediates the delivery of mRNA into human primary keratinocytes and in vivo |
| Peptide | PepFect14 (PF14) |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | ~25.6 µM |
| Rna Concentration | ~850 ng per injection |
| Mixing Ratio | CR 2:1 |
| Formulation Format | peptide–mRNA nanoparticles |
| Formulation Components | PF14 ± CaCl2 / PS80 |
| Size Nm | 100.00 |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Mouse irritant contact dermatitis (ear skin) |
| Administration Route | subcutaneous injection |
| Output Type | reporter expression in tissue |
| Output Value | EGFP expression detected in skin; no inflammation |
| Output Units | |
| Output Notes | No ear swelling; no cytokine induction |
| Toxicity Notes | Well tolerated |
| Curation Notes |