Sequence: RRRRRRRR (octa-arginine) functionalized on hydroxyapatite NP
| Experiment Id | EXP002111 |
|---|---|
| Paper | Design of a Biocompatible Hydroxyapatite-Based Nanovehicle for Efficient Delivery of Small Interfere |
| Peptide | R8HNPs |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 100 µg/mL (NP) |
| Rna Concentration | 100 nM |
| Mixing Ratio | 100 µg/mL NP : 100 nM siRNA |
| Formulation Format | CPP-functionalized inorganic nanoparticle |
| Formulation Components | Hydroxyapatite NP + octa-arginine (R8) |
| Size Nm | 40.00 |
| Zeta Mv | 2.55 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | R1 mouse embryonic stem cells |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | ~80% Oct4 mRNA knockdown; ~97% protein knockdown at 48 h |
| Output Units | |
| Output Notes | Functional siRNA delivery demonstrated in vitro only; no animal studies |
| Toxicity Notes | No significant toxicity ≤100 µg/mL |
| Curation Notes |