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EXP002118

Paper

Discovery of peptides for ligand-mediated delivery of mRNA lipid nanoparticles to cystic fibrosis lung epithelia (2024)

Peptide

Peptide C

Sequence: CTSTRKKQC

RNA

mRNA

All experiment fields

Experiment Id EXP002118
Paper Discovery of peptides for ligand-mediated delivery of mRNA lipid nanoparticles to cystic fibrosis lu
Peptide Peptide C
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration
Mixing Ratio N/P = 6
Formulation Format peptide-lipid functionalized LNP
Formulation Components SM-102/DSPC/Cholesterol/DMG-PEG + peptide-lipid
Size Nm 80.00
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model BALB/c and Ai9 mice
Administration Route intratracheal
Output Type reporter expression / genome editing
Output Value 3.4-fold higher lung luciferase vs Spikevax; epithelial tdTomato expression
Output Units
Output Notes Robust lung-localized mRNA expression and Cre-mediated genome editing
Toxicity Notes No significant toxicity in vitro or in vivo
Curation Notes