Sequence: CRGDKGPDC-GRKKRRQRRRC
| Experiment Id | EXP002119 |
|---|---|
| Paper | Dual-mRNA Delivery Using Tumor Cell Lysate-Based Multifunctional Nanoparticles as an Efficient Colon |
| Peptide | TAT-iRGD |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 10 μg per dose (each mRNA) |
| Mixing Ratio | 25:1 (CLSV:mRNA, w/w) |
| Formulation Format | CPP-functionalized polymer-lipid nanoparticle |
| Formulation Components | DOTAP-mPEG-PCL + tumor cell lysate + TAT-iRGD (fCPP) |
| Size Nm | 241.00 |
| Zeta Mv | 39.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | CT26, 293T cells; BMDCs |
| Animal Model | |
| Administration Route | |
| Output Type | mRNA expression / apoptosis / immune activation |
| Output Value | High EGFP/mCherry expression; strong Bim/IL-23A expression; apoptosis induction |
| Output Units | |
| Output Notes | Efficient cytosolic mRNA delivery with functional protein expression in vitro |
| Toxicity Notes | No significant systemic or cellular toxicity observed |
| Curation Notes |