Targeting Listeria monocytogenes consensus sequence of internalin genes using an antisense molecule (2019)
Sequence: RFRRKRRKRRKRRKRK
| Experiment Id | EXP002121 |
|---|---|
| Paper | Targeting Listeria monocytogenes consensus sequence of internalin genes using an antisense molecule |
| Peptide | Arg-rich CPP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 8–32 µM |
| Rna Concentration | 8–32 µM (CPP-PNA) |
| Mixing Ratio | 1:1 (CPP:PNA, covalent) |
| Formulation Format | CPP–PNA conjugate |
| Formulation Components | Arginine-rich CPP + antisense PNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Caco-2, HeLa cells; Listeria monocytogenes (4b) |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown / invasion inhibition |
| Output Value | 3–5.6-fold internalin mRNA knockdown; ~55–59% invasion reduction |
| Output Units | |
| Output Notes | Functional antisense delivery demonstrated in bacterial and cell culture models only |
| Toxicity Notes | Low cytotoxicity; minimal apoptosis and cytokine induction |
| Curation Notes |